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KMID : 0355620080340060602
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Journal of Korean Association of Oral and Maxillofacial Surgeons
2008 Volume.34 No. 6 p.602 ~ p.610
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ACRYLAMIDE-INDUCED NEOPLASTIC TRANSFORMATION OF HUMAN EPITHELIAL CELLS IN CULTURE
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Kim Sang-Kyu
Kim Jin-Wook
Kim Chin-Soo
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Abstract
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Purpose: Acrylamide is present in significant quantities in a wide range of commonly consumed human foods. Carcinogenic risk of acrylamide through the consumption of food is a great public concern and in controversy, but it is not properly addressed due to the lack of evidence in humans. While a plenty of data is available on the carcinogenicity in animal models, the studies in humans are limited. Thus, the present study attempted to examine the carcinogenic potentials of acrylamide on the human epithelial cell, which is the target cell origin of the most cancers.
Material and method & Result: 1. Acrylamide was not cytotoxic up to 100 μM as measured by MTT and LDH assays, indicating a relatively low toxicity of this substance in human epithelial cells.
2. The parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of acrylamide.
3. The neoplastic transformation was further increased with the co-treatment of TPA
4. Antioxidants blocked the generation of Reactive Oxygen Species(ROS) and the GSH depleting agent dramatically increased the ROS production.
5. mRNA levels of fibronectin following acrylamide exposure was increased in a dose-dependent manner, indicating a possible biomarker of acrylamide- induced cellular transformation.
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KEYWORD
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Acrylamide, carcinogenic potential, Neoplastic transformation, Human epithelial cell
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